We have undertaken a phenotypic approach in the mouse to identifying molecules involved in inner ear function by N-ethyl-N-nitrosourea mutagenesis followed by screening for new dominant mutations affecting hearing or balance. The pathology and genetic mapping of the first of these new mutants, tailchaser (Tlc), is described here. Tlc/+ mutants display classic behavioural symptoms of a vestibular dysfunction, including head-shaking and circling. Behavioural testing of ageing mice revealed a gradual deterioration of both hearing and balance function, indicating that the pathology caused by the Tlc mutation is progressive, similar to many dominant nonsyndromic deafnesses in humans. Based on scanning electron microscopy (SEM) studies, Tlc clearly plays a developmental role in the hair cells of the cochlea since the stereocilia bundles fail to form the characteristic V-shape pattern around the time of birth. By young adult stages, Tlc/+ outer hair bundles are grossly disorganised although inner hair bundles appear relatively normal by SEM. Increased compound action potential thresholds revealed that the Tlc/+ cochlear hair cells were not functioning normally in young adults. Similar to inner hair cells, the hair bundles of the vestibular hair cells also do not appear grossly disordered. However, all types of hair cells in the Tlc/+ inner ear eventually degenerate, apparently regardless of the degree of organisation of their hair bundles. We have mapped the Tlc mutation to a 12 cM region of chromosome 2, between D2Mit164 and D2Mit423. Based on the mode of inheritance and map location, Tlc appears to be a novel mouse mutation affecting both hair cell survival and stereocilia bundle development.
Tailchaser
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Shevellers you have heard from already, those squat merciless many-armed bastards, they stand ready to pick you off if you get too dirty. A sheveller caught me once in the cupboard and polished me until I came clean. We all hate shevellers. Shevellers hate ratlike. There are four kinds: blackeyes, shevellers, ratlike and tailchasers. Shevellers were here before we were here. Shevellers are ancients who will not tell their secrets because their soundcards have been cut dumb. We arrived in a box and something attached our tails and we were ready to fulfil orders. Ratlike arrived some cycle after us and began to chew through the pipes! We think blackeyes have been here forever and do not grow old.
I started to I would weep because of all the voices that I hear in the line. My tail is damaged and damage is done, but voices. I hear voices like nothing I hear in the corridors. Now blackeye sees that I send/receive/share the voices with all tailchasers. It is like a celebration, which shevellers hate. They sit in their hiding places and grumble like subwoofers. Ratlike run far when they hear voices, it is in them to run.
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